Analysis Note

Single band by SDS-PAGE

Biochem/physiol Actions

Primary TargetTetanus toxin is one of the deadliest toxins known

Product does not compete with ATP.

Binds G_T1b ganglioside in a hemagglutination assay

General description

Tetanus toxin is produced by Clostridium tetani. Composed of a 100 kDa heavy and a 50 kDa light chain joined by a single interchain disulfide bond.

Produced by the anaerobic bacillus Clostridium tetani, Tetanus toxin is one of the deadliest toxins known. Composed of two polypeptide chains joined by a single interchain disulfide bond. The heavy chain (~100 kDa) participates in binding to the nerve terminals, internalization, retrograde axonal transport, and membrane translocation of the light chain (~50 kDa) into the neuronal cytosol. The light chain inhibits neurotransmitter release after reduction of the disulfide bridge.

Produced by the anaerobic bacillus Clostridium tetani. Tetanus toxin is one of the deadliest toxins known. Composed of two polypeptide chains joined by a single interchain disulfide bond. The heavy chain (100 kDa) participates in binding to the nerve terminals, internalization, retrograde axonal transport, and membrane translocation of the light chain (50 kDa) into the neuronal cytosol. The light chain inhibits neurotransmitter release after reduction of the disulfide bridge.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Montecucco, C., et al. 1994. FEBS Lett. 346, 92.Wellhöner, H.H., et al. 1987. J. Biol. Chem. 262, 17374.

Packaging

25 µg in Alu drum

Reconstitution

Please refer to the vial label for lot-specific reconstitution volume.

Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 1 month at 4°C.

Warning

Toxicity: Highly Toxic (H)